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Fig. S8
4C4 immunohistochemistry labels virtually all mpeg1+ tectal cells and some mpeg1+ skin cells, but not mpeg1+ cells in the trunk. (A,C,E) Confocal images of the optic tectum (A), skin (C) and trunk (E) of a mpeg1:GFP animal after 4C4 and GFP immunohistochemistry. Filled or empty arrows indicate colocalisation, or lack thereof, between 4C4 and mpeg1. Scale bars, 50 μm (A,E) and 20 μm (C). (B,D,F) Quantification of the proportion of 4C4+/mpeg1+ cells among all mpeg1+ cells in the tectum (B), skin (D) and trunk (F). n ≥ 5 animals per experimental group
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