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Fig. S2
Cell death after brain injury can be detected through Annexin A5 live imaging, caspase 3 immunohistochemistry, and TUNEL staining. (A,C,E) Confocal images of the tectum of a NBT:secA5-BFP animal (A), and animals after cleaved caspase 3 immunohistochemistry (B) or TUNEL staining (C), at different time points after injury. Scale bars, 50 μm. (B,D,F) Quantification of Annexin A5+ cells (B), cleaved caspase 3+ cells (D) or TUNEL+ cells (F). n ≥ 6 animals per experimental group. *, p < 0.05 and **, p<0.01 in two-way ANOVA
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