ZFIN Image: Dohaku et al., 2019, Figure 3

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Figure Caption

Figure 3

Transgenic lines for tracing Purkinje cell (PC) afferents. (A) Schematic drawing of the Tol2 plasmids used to express TVA-mCherry or rabies virus glycoprotein (G). The approximately 5-kbp aldoca promoter was used to express transgenes specifically in PCs. To identify Tg fish harboring the G transcription unit, the Tol2 vector pBH (Bleeding Heart vector), which expresses mCherry in the heart was used. (B,C) Immunostaining of sagittal sections of the adult Tg(aldoca:TVA-mCherry) fish cerebellum with anti-mCherry (anti-DsRed, magenta) and anti-parvalbumin7 (anti-Pvalb7, green) antibodies. (C) High-magnification images of the box in (B). TVA-mCherry (Ba,Ca), Pvalb7 (Bb,Cb), and merged images (B,C) are shown. PCs that expressed TVA-mCherry also expressed Pvalb7. Scale bars: (B) 100 μm, and (C) 20 μm.

Figure Data

Constructs: Tg(aldoca:EnvA,myl7:mCherry) , Tg(aldoca:Tva-mCherry)

Acknowledgments

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