ZFIN Image: Dohaku et al., 2019, Figure 4

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Figure Caption

Figure 4

Transgenic lines for tracing GC afferents. (A) Schematic drawing of the Tol2 plasmids used to express TVA-mCherry or RVG. The cbln12 promoter was used to express transgenes in GCs. pBH was used to identify G-expressing fish. (B,C) Immunostaining of sagittal sections of the adult Tg(cbln12:TVA-mCherry) cerebellum with anti-mCherry (magenta) and anti-Neurod1 (green) antibodies. (C) High-magnification images of the box in (B). TVA-mCherry (Ba,Ca), Neurod1 (Bb,Cb), and merged images (B,C) are shown. Note that TVA-mCherry was located on the cell membrane of Neurod1⁺ GCs (C). Scale bars: (B) 50 μm, and (C) 5 μm.

Figure Data

Constructs: Tg(cbln12:Tva-mCherry) , Tg(cbln12:EnvA,myl7:mCherry)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neural Circuits