Figure 3

Figure 3

Zebrafish hypocretin is associated with arousal. (A) Dorsal view of a 4 dpf zebrafish larva that expresses GFP-Aequorin (GA) specifically in Hcrt neurons. (B) Two-photon z-projection image of the boxed area in (A). Scale bars represent 100 μm (A) and 50 μm (B). (C) Overexpression of Hcrt using a heat shock-inducible promoter (HS-Hcrt) increases locomotor activity. The mean locomotor activity of 20 HS-Hcrt larvae and 20 of their wild-type siblings is shown. The spike in activity during the afternoon of the 2nd and 3rd days of the experiment resulted from the addition of water to offset evaporation. (D) The GA assay. A large-area photon-counting photomultiplier tube is placed above a transparent behavior chamber in which a zebrafish larva that expresses GA in specific neurons is allowed to freely swim. The larva is imaged using infrared (IR) lights and an IR camera that is placed below the recording chamber. Spectral separation between GA neuroluminescence and the IR illumination allows the simultaneous recording of GA neuroluminescence and larval behavior. (E) Activity of Hcrt neurons during natural behavior. Data for a representative 4 dpf larva is shown. The larva exhibited periods of increased spontaneous locomotor activity (lower trace, thick line indicates 10 min running average) during the subjective day (hatched bar below graph) and little activity during the subjective night (black bar below graph). Most neuroluminescence signals produced by Hcrt neurons (upper trace) coincide with periods of robust locomotor activity during the subjective day, suggesting that Hcrt neuron activity is associated with arousal. Figure modified from Prober et al. (2006), Naumann et al. (2010).