ZFIN Image: Budi et al., 2019, Fig. 5

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Figure Caption

Fig. 5

Effects of Insulin and SB431542 on Intersomitic Vessels in Zebrafish Embryos

(A) Scheme representing the treatment protocol. Tg(fli:gfp)zebrafish embryos were treated for 72 h.

(B) Expression of pck1 mRNA was quantified by qRT-PCR. RNA was extracted from pool of larvae that had received insulin with DMSO or DMSO control for 48 h.

(C) Altered blood vessels in insulin- and SB431542-treated embryos. Representative images show the trunk vasculature of 96 hpf tg(fli:EGFP) zebrafish larvae. The boxes mark the region shown at higher magnification in the lower right corner of the panel.

(D) Branching quantification of zebrafish blood vessels at 96 hpf, as seen in C in control solution DMSO, SB431542, insulin, and insulin + SB431542. Bars represent mean ± SD. Zf, zebrafish. Ctrl, control.

Figure Data

Phenotype details

Acknowledgments

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