ZFIN Image: Cau et al., 2019, Fig. 7

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Figure Caption

Fig. 7

Activation of BMP signaling at different times affects PhR subtype identity.

(A) Scheme of the experimental design showing the timing of the heat shocks and analysis of the number of exorh+ and PT+ cells. (B-G) In situ hybridization for exorh and PT in 48-hpf WT and Tg(hsp70:bmp2b)^fr13 transgenic embryos (BMP GOF) heat shocked at 10 or 16 hpf. Embryos are viewed dorsally with anterior to the top. Scale bar represents 10 μm. (H-I) Counts of exorh+ and PT+ cells in WT and Tg(hsp70:bmp2b)^fr13embryos after a heat shock performed at various stages (indicated on the x-axis). Error bars represent SD. *p < 0.05. **p < 0.001. ***p < 0.0005. ****using a Kruskal-Wallis test with Dunn’s post hoc comparisons of the transgenic versus WT populations. Tg(hsp70:bmp2b)^fr13 transgenic embryos were labeled BMP GOF. Underlying data can be found in S1 Data. BMP, bone morphogenetic protein; exorh, exorhodopsin; GOF, gain of function; hpf, hours post fertilization; PhR, photoreceptor; PT, parietopsin; WT, wild type.

Acknowledgments

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