ZFIN Image: Otis et al., 2019, Fig. 2

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Figure Caption

Fig. 2

Dietary TF-cholesterol localizes to the endosomal-lysosomal trafficking network in enterocytes. Representative images show that 3 h following the onset of feeding, dietary TF-cholesterol colocalizes to mCherry-Rab5c (early endosomes) (A), mCherry-Rab11a (recycling endosomes) (B), and mCherry-Rab7 (late endosomes) (C). n = 3, with 3–9 fish per n; all fish 6-dpf. TF-cholesterol colocalizes with BODIPY TR ceramide, which marks the trans-Golgi network, in enterocytes after 4 h of feeding (D). n = 3, with 3–6 fish per n. Arrows indicate colocalization; dpf, days postfertilization; L, intestinal lumen; N, nucleus; TF-cholesterol, TopFluor-cholesterol.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Gastrointest. Liver Physiol.