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Fig. 5 Three Loci Associated with Heart Regeneration Identified by QTL Analysis (A) The second generation of a cross between surface fish and Pachón results in offspring ranging from large, pigmented fish with no eyes to small cavefish-like fish with black eyes. One hundred eighty-eight F2 hearts were isolated 90 days after resection. (B) Number of F2 generation hearts per heart regeneration class. (C) AFOG staining on F2 hearts. Examples of four of the seven groups used for correlation tests and QTL analysis. Group 0, clear scar, neither compact wall myocardial thickening nor overgrowth (arrowhead). Group 2, clear scar, compact myocardium has started to grow over the scar (arrowhead). Group 4, clear scar, compact myocardium closed. Group 6, no sign of injury left. (D) No correlation between body melanocyte number (r = 0.085) or eye size (r = 0.089) and heart regeneration. Pearson’s correlation. (E) LOD scores per LG, showing three loci above the genome-wide significance threshold of 2.82 (95th percentile of maximum LOD from permutation analysis). (F) Circos plot mapping markers within LOD score peaks on LG1, LG9, and LG10 to the cavefish genome contigs and showing significant log2 fold expression changes for genes overlapping those loci. Detailed numbers and statistics are provided in STAR Methods. All scale bars, 100 μm.