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Fig. 1
PKC overactivation during early development causes mild developmental delay and brain size deficit.
a Lateral views of representative larvae (3 dpf) exposed to DMSO, PMA or PMA+Go6983. Head size was calculated with the boundary shown in yellow dotted line. b Quantitative representation of body length and head size (3 dpf) normalized to DMSO (DMSO n = 8, PMA n = 10, PMA+Go6983 20 nM n = 6, PMA+Go6983 100 nM n = 10, **p < 0.01, ***p < 0.001, one-way ANOVA). c Lateral views of representative larvae (9 dpf) previously exposed to DMSO or PMA. Head size was calculated with the boundary shown in yellow dotted line. d Quantitative representation of body length and head size (9 dpf) normalized to DMSO (DMSO n = 8, PMA n = 6, *p < 0.05, t-test analysis). e Dorsal views of zebrafish brains (3 dpf) visualized by Huc:GFP. Brain region was divided by yellow dotted line. Scale bar, 100 μm. f Quantitative representation of different brain regions normalized to DMSO (n = 16 per group, ***p < 0.001, t-test analysis). g DAPI staining of zebrafish head sections (3 dpf). Scale bar, 100 μm. h Nuclei number in forebrain and midbrain (n = 6 per group, ***p < 0.001, t-test)