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Fig. 6
Two-color fluorescent imaging of whole mount SG-WT zebrafish (Danio rerio) treated with Os(II) sulfonamide 6 for 96 h. Fluorescence for ROS (green) and 6 (red) is shown superimposed onto bright field images. Overlapping regions (yellow) are shown. Confocal images were acquired using a Zeiss LSM880 confocal microscope. Embryos were stained using the green reagent of the ROS/Superoxide Detection Kit (Enzo life sciences) for ROS detection. Excitation, 458, 488, and 561 nm; green emission for ROS, 493–550 nm; red emission for 6, 568–750 nm. See SI for full confocal data.
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Full text @ J. Med. Chem.