IMAGE

Fig. S5

ID
ZDB-IMAGE-181206-20
Source
Figures for Xu et al., 2018
Image
Figure Caption

Fig. S5

TBK1/IKKε suppression promotes b-cell regeneration via PKA and mTOR signaling axis.

(A-L) Bright-field images combined with fluorescent images showing the overall morphology and [Tg(ins:CFP-NTR)s892; Tg(ins:Kaede)jh6] expression (green) of larvae at 48 hpa treated with DMSO (A), calcineurin inhibitor FK506 (B), mTOR inhibitor rapamycin (C), PKA inhibitor PKI-(6-22)-amide (D), PI3K inhibitor LY294002 (E), Akt inhibitor MK2206 (F), PIAA (G), and combinations of each inhibitor with PIAA (H-L), respectively. Treatment of rapamycin and PKI-(6-22)-amide suppressed PIAA-mediated b-cell regeneration (G vs. I and J). The individual LY294002 and MK2206 caused increase in the [Tg(ins:CFP-NTR)s892; Tg(ins:Kaede)jh6]-expressing cell population during regeneration (E and F). The combinations of LY294002 or MK2206, especially MK2206, with PIAA resulted in significant increase in b-cell regeneration (K and L) compared to individual treatments (E and F). The insets display magnified views of the pancreatic islets (outlined by the dashed squares). (M-N) Quantification of the number (mean±SD) of total regenerated b-cells at 48 hpa (in A-L; 4.7±1.2 (DMSO), 5.5±1.7 (FK506), 4.2±1.3 (rapamycin), 5.4±1.7 (PKI-(6-22)-amide), 8.7±1.7 (LY294002), 14.8±2.7 (MK2206), 17.9±2.4 (PIAA), 16.7±2.3 (PIAA with FK506), 7.0±2.1 (PIAA with rapamycin), 9.6±2.8 (PIAA with PKI-(6-22)-amide), 21.3±4.3 (PIAA with LY294002), 31.9±5.1 (PIAA with MK2206)). Cells in 20 planes of confocal images from 20 individual larvae were counted per condition. **, P < 0.01; ***, P < 0.001. (O) A model depicting the role of PKA and mTOR signaling pathways in mediating the b-cell regeneration response to TBK1/IKKε inhibition. The site of action of PIAA is shown in red, while those of LY294002 and MK2206 in blue.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.