IMAGE

Fig. 3

ID
ZDB-IMAGE-181030-11
Source
Figures for Yang et al., 2018
Image
Figure Caption

Fig. 3

Neural crest cells are in close contact with pancreatic endocrine cells early in development.

(A) Lineage tracing of neural crest cells in Tg(sox10:CreERT2, myl7:GFP); Tg(ubb:loxP-CFP-loxP-nuc-mCherry); Tg(elavl3:GAL4-VP16); Tg(UAS:EGFP-CAAX) zebrafish following 5 µM tamoxifen treatment from 16 to 24 hpf and staining at 35 hpf. (B) Whole mount immunostaining at 35 hpf for mCherry (neural-crest-derived cells), GFP (elavl3-positive cells), Insulin (beta cells), and DAPI (DNA). (C) Confocal imaging of Tg(sox10:GAL4); Tg(UAS:GFP); Tg(sst2:RFP) zebrafish mounted in 0.5% agarose from 23 to 33 hpf. Yellow arrow points to a neural crest cell in close proximity to endocrine pancreatic cells and briefly contacting islet cells before migrating away. (D) Whole mount immunostaining at 34 hpf for GFP (sox10-positive cells), Insulin (beta cells), RFP (delta cells), and DAPI (DNA). Yellow arrows point to neural-crest-derived cells that were once in contact with the pancreatic islet. (E) Whole mount immunostaining at 34 hpf for GFP (sox10-positive cells), HuC/HuD (mature neurons), RFP (delta cells), and DAPI (DNA). Yellow arrows point to neural-crest-derived cells that were once in contact with the pancreatic islet.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife