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Fig. S7
Proliferation is necessary for cell height increase.
(A) Cell height of live (light sheet time lapses) Rockout-treated wild-type retinal PSE (gray). Sample was incubated in 175 μM Rockout for the entirety of the imaging session. Around 37 hpf, the basolateral actin accumulation was abolished, nuclei filled the basal positions, and proliferation stopped. Related to S6 Movie. (B) Cell height of live (light sheet time lapses) wild-type retinal PSE, treated with a combination of cell cycle inhibitors HU/A (red); 30 mM HU and 150 μM of A were added at the beginning of the movie (30 hpf). Proliferation stopped 3 h later (red arrowhead). Cell height did not increase further after cells cycle was blocked. Control plots (blue) in (A) and (B) are data from Fig 6E. (C) Top panels: Retinas of mutant fish treated with DMSO fold and proliferate normally (PH3 staining). Bottom panels: Mutant retinas treated with HU/A do not fold when their proliferation is inhibited but do maintain the basolateral actin accumulation. Scale bar: 50 μm. (Underlying data can be found at DOI: 10.5281/zenodo.1316912; /Matejcic-et-al_2018/Data/FS7A.csv and FS7B.csv.). A, aphidicolin; hpf, hours post fertilization; HU, hydroxyurea; PH3, phosphorylated histone H3; PSE, pseudostratified epithelium.