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Fig. 1
One Zebrafish PITPNC1 Ortholog Is Exclusively Expressed in the Brain
(A) Zebrafish PITPNC1 orthologs and human splice variants.
(B–D′) pitpnc1a mRNA is detected in the nervous system at 24 hpf (B and B′), 48 hpf (C and C′), and is widespread in the brain by 5 dpf (D and D′).
(E–G′) pitpnc1b mRNA is expressed in non-neuronal tissue, including the pharyngeal arches and olfactory pits (E–F′). pitpnc1b mRNA is undetectable in the larval brain (G and G′).
(H) pitpnc1a mRNA is expressed in many areas of the adult zebrafish brain.
(I) Pitpnc1a protein is detected only in the adult zebrafish brain. Transfected HEK293 cells are a positive control.
Tel, telencephalon; TeO, optic tectum; Hyp, hypothalamus; Hb, hindbrain; Hab, habenula; Cb, cerebellum. Scale bars, 100 μm (B–G′) and 500 μm (H).