Fig. 6

Effect of cigarette smoke extract in adult zebrafish vascular regeneration. Adult Tg(fli1a:EGFP)^y1 zebrafish were exposed to a 0.25% dilution of CSE for ten days before, and 10 days after tail fin amputation. The rate of revascularization was calculated using the signal of the fin fluorescent endothelial cells and normalized to the total regenerated area. While exposure to CSE limited fin regrowth in the treated group, re-vascularization of the fin was not affected. Results were obtained from three independent experiments using a total of N ≥ 20 zebrafish for each condition. *p < 0.05. Scale bars represent 1 mm.