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Fig. 4
Effect of cigarette smoke on adult zebrafish survival and regeneration potential. The lethal effect of CSE was studied in 6–12 month adult zebrafish exposed to increasing dilutions of cigarette smoke extract for 10 days. Results represent three independent experiments, using a total of N ≥ 15 zebrafish for each condition (A). Zebrafish, which underwent tail fin amputation showed diminished regeneration capacity, when exposed to a pre- and post-conditioning treatment with non-lethal concentrations of CSE (0.25%, 20-day treatment in total), as shown by the diminished regenerated fin tissue compared to non-treated condition. Results were obtained from three independent experiments, using a total of N ≥ 25 adult zebrafish for each condition (B,C). *p < 0.05. Scale bar represents 1 mm.