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Fig. 3
Effect of cigarette smoke on zebrafish vascular development. An impairment of intersegmental vessel (ISVs) formation and extension in one-day old transgenic Tg(fli1a:EGFP)y1 embryos was observed upon exposure to increasing CSE-concentrations for 24 hours. The number of anastomosed ISVs was quantified as a parameter for vessel maturation for the five anterior vessels proximal to the urogenital opening for each CSE-condition. Results were obtained from three independent experiments, using a total of N ≥ 90 larvae for each condition. *p < 0.05. Scale bars represent 100 μm.
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