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Fig. 2-S1
Purification of TRPC4DR and negative stain EM of TRPC4DR in amphipols.
(a) SDS-PAGE of the peak fraction of TRPC4DR in amphipols after size exclusion chromatography. Lane 1: molecular weight marker, lane 2: protein. (b) Size exclusion chromatography profile of TRPC4DR in amphipols. Peak 1, 2 and 3 correspond to the void volume, tetrameric TRPC4DR and cleaved GFP, respectively. (c) Representative negative stain electron micrograph of TRPC4DR. Scale bar, 50 nm, (d) Representative 2-D class averages. Scale bar, 10 nm. (e) 3-D reconstruction of negatively stained TRPC4DR shown in different orientations.
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