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Fig. 1

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ZDB-IMAGE-180711-25
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Figures for Selland et al., 2018
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Figure Caption

Fig. 1

Hoxb1bua1006 and hoxb1asa1191 mutations.

(A) The hoxb1bua1006 mutation was generated via TALEN-mediated mutagenesis and contains a 13 bp insertion 63 bp downstream of the start codon. This mutation results in a truncated 43aa protein. (B) The hoxb1asa1191 mutation contains a G to A resulting in a truncation part way through the homeodomain at amino acid 269. To determine if the protein produced by the hoxb1bua1006 allele retains any function, 200 pg of wildtype hoxb1b RNA (D) and 200 pg of mutant hoxb1b RNA containing the ua1006 mutation (E) were over expressed. In situ hybridization for the 3’UTR of hoxb1a in uninjected controls (C,F) shows normal expression in rhombomere 4. Overexpression of wildtype hoxb1b RNA results in a homeotic transformation where a duplicated region hoxb1a expression is observed in r2 (66/88 embryos affected) (D). Overexpression of hoxb1bua1006 RNA fails to cause a homeotic transformation, indicating a lack of biological activity (0/82 embryos affected) (E). Similarly, to determine if the protein produced by the hoxb1asa1191 allele retains any function, 50 pg of wildtype hoxb1a RNA (G) and 50 pg of mutant hoxb1b RNA containing the sa1191 mutation (H) were over expressed. Overexpression of wildtype hoxb1a RNA results in a homeotic transformation where a duplicated region hoxb1a expression is observed in r2 (30/34 embryos affected) (G). Overexpression of hoxb1asa1191 RNA fails to cause a homeotic transformation, indicating a lack of biological activity (0/38 embryos affected)(H). All embryos are dorsal views, anterior to the left, 18hpf.

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Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.