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Fig. S2
Effect of IMD0354 treatment in zebrafish embryos and systemic effects in adult rat liver.
(A) In vivo images of zebrafish embryos 24 hpf, treated with IMD0354 (0, 1, 10, 100 and 200 ng/ml).
(B) In vivo images of zebrafish embryos 72 hpf, treated with IMD0354 (0, 1, 10, 100 and 200 ng/ml).
(C) Mortality rate of zebrafish embryos treated with IMD0354 (0, 1, 10, 100 and 200 ng/ml) at 24 and 72 hpf. (n=40) One-way ANOVA test with Tukey multiple comparisons were used to determine statistical significance. n.s. p>0.05; *** p<0.001
(D) Hematoxylin and Eosin (H&E), (E) Cleaved-Caspase 3 (green); Nuclear counterstaining by DAPI (blue) in fluorescent images.