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Fig. 3
Overview of lateral line system. (A) Lateral view of 6 dpf larva with neuromasts of anterior and posterior lateral line system. Schematic drawing of neuromasts viewed from lateral. (A1) Approximate location of trigeminal ganglion (TG), anterior (AG) and posterior lateral line ganglion (PG): gray. Colors and abbreviations of head neuromasts described below (B,C), lateral branch of posterior lateral line (maroon): L1–L8, secondary lateral branch (light brown): LII.1–LII4. (A2) Lateral view of montage of seven optical stacks taken with fluorescence microscope of live 6 dpf larva stained with DASPEI in vivo to visualize neuromasts. Schematic as shown in (A1) overlaid in magenta, neuromasts marked in green. Location of trigeminal and lateral line ganglia marked with blue asterisks. Landmark structures labeled: olfactory bulb (OB) also slightly stained, otic capsule (OC). Depth of stacks: I: 62 μm, II: 250 μm, III: 82 μm, IV: 134 μm, V: 160 μm, VI: 110 μm, VII: 34 μm, step size: 2 μm. (B). Dorsal view of 6 dpf larval head with neuromasts of the anterior lateral line system as well as dorsal and medial branch of posterior lateral line system. (B1) Schematic drawing of anterior and dorsal lateral line neuromasts relative to anatomical landmarks. Dorsal branch (red) D1–D3 and medial branch (magenta) MI1 and MI2, all with cell bodies of their afferent neurons in the posterior/medial-posterior lateral line ganglion. Otic branch (blue) O1 and O2; Infraorbital branch (dark green) IO4; supraorbital branch (light green) SO1–SO3; nasal branch (orange) N1 and N2; and mandibular branch (cyan) M1, all with cell bodies of their afferent neurons in the anterior lateral line ganglion. (B2) Dorsal view of Z-MIP of montage of two tiles of rostral part of a whole mount of entire larva (total depth: 477 μm, step size: 1 μm) with overlaid schematic as shown in (B1) eGFP-CAAX/SypGFP expression driven by th:Gal4-VP16 visualized by immunofluorescence in green, TOTO-3 labeling of cell nuclei in magenta. (C) Ventral view of 6 dpf larval head with neuromasts of the anterior and otic lateral line. (C1) Schematic drawing relative to anatomical landmarks. MI1–MI2 (magenta), O1–O2 (dark blue), with cell bodies of innervating afferents in the posterior/medial-posterior lateral line ganglion. Opercular neuromast (dark red): OP, infraorbital branch (dark green): IO1–IO4, N1–N2 (orange), mandibular branch (cyan): M1–M2, with cell bodies of innervating afferents in the anterior lateral line ganglion. (C2) Ventral view of Z-MIP of montage of two tiles of rostral part of a whole mount of entire larva (total depth: 434 μm, step size: 1 μm) with overlaid schematic as shown in (C1). Pseudo colors as in (B).