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Fig. 6
TUNEL assay for the tbx5 morphants and IGF-I-treated tbx5 morphants. TUNEL assay revealed no TUNEL-positive cells in the WT (a), MIS (b), and WTIGF1 (c) groups. However, massive numbers of TUNEL-positive cells were visible in the MO group (d). In the MOIGF1 group, the amount of TUNEL-positive cells was reduced (e). Most of the TUNEL-positive cells were clustered over the tail and trunk, while several were be found over the yolk sac and head (f). Arrow, TUNEL-positive cells. Data are presented as the means ± standard deviations. WT: wild-type embryos; MO: tbx5 morphants; MIS: mismatch tbx5 morpholino-treated wild-type embryos; WTIGF1: IGF-I-treated wild-type embryos; MOIGF1: IGF-I-treated tbx5 morphants. The number of specimens was 10, and the number of independent experiments was 3 in each group