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Fig. 2
MTZ treatment eliminating the PGCs in the transgenic embryos. The PGCs (white arrow) marked with mCherry fluorescent were around anterior part of the yolk extension in Tg(nanos3:nfsB-mCherry-nanos3 3′UTR) transgenic embryos at 24 hpf (A), and were almost arranged in the genital ridge at 36, 48, 60, and 72 hpf (C,E,G,I), respectively. After treated with MTZ, the 24 hpf embryos exhibited the decreased number of PGCs with the weaker fluorescence (B). The number of PGCs was fewer and the fluorescent signal became weaker in the MTZ-treated embryos at 36 hpf (D). At 48 hpf, the fluorescence labeled-PGCs almost disappeared (F). No fluorescence labeled-PGCs were observed in the MTZ-treated embryos at neither 60 hpf (H) nor 72 hpf (J).