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Fig. 6
Transgenic zebrafish lines (ET33-10, ET27) express GFP in the SCO, which represents the anterior bulging of the midbrain roof plate. The SCO expression detected by WISH (magenta, A) or by confocal microscopy in vivo (green-B,B'). Sco-spondin forms the Reissner fiber detected by anti-Reissner fiber antibody (red, B'). All images are of whole mounts in lateral view (anterior to the left). (A) WISH; (B) confocal microscopy in vivo; (B') two-color immunohistochemistry; (A) ET33-10; (B,B') ET27. cpIII, choroid plexus (IIIrd ventricle); d, diencephalon; e, epiphysis (pineal); h, hour postfertilization; mhb, midbrain-hindbrain boundary; mrp, midbrain roof plate; ot, optic tectum; rf, Reissner fiber; sco, subcommissural organ; t, telencephalon. Scale bar−50 μm.