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Fig. S1
Predominant expression in the YSL after 4-cell yolk mRNA injection. A. MZnanog embryos were injected with 33 pg GFP mRNA and 25 pg nanog mRNA at 1-cell stage, 4-cell stage in vegetal yolk, or in 1 of the cells at 16-cell stage. At sphere (4 hpf) stage, embryos were imaged and scored for spatial GFP expression. B. Representative rescued embryos (as indicated) were imaged at 24 hpf for GFP expression in embryonic tissues. C. In situ hybridization for mxtx2 and ndr2 expression at 4.5 hpf (dome / 30% stage) in wild-type, MZnanog, and MZnanog embryos injected with nanog mRNA into the vegetal yolk at the 4-cell stage.