IMAGE

Fig. 2

ID

ZDB-IMAGE-180417-20

Antibodies

Ab9-smad2

Source

Figures for Rogers et al., 2017

Image

Figure Caption

Fig. 2

Lefty loss causes expanded Nodal signaling gradients.

α-pSmad2 staining (red) at sphere stage (A, animal views) and 50% epiboly (B, lateral views) in representative wild type and lft1^-/-;lft2^-/- mutant embryos (nuclei labeled with Sytox nuclear stain (green)). Note stronger pSmad2 staining in lft1^-/-;lft2^-/- mutant embryos compared to wild type. (C) Quantification of pSmad2 gradients in wild type (blue) and lft1^-/-;lft2^-/- (red) mutant embryos at dome stage (dashed) and 50% epiboly (solid) shows an increase in the amplitude and range of the signaling gradient in lft1^-/-;lft2^-/- mutants. See Materials and methods and Figure 2—figure supplement 1 for quantification details.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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