ZFIN Image: Sawamiphak et al., 2017, Fig. S9

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Figure Caption

Fig. S9

Jam3b deficiency impedes FATC activation in skeletal muscles. A-P, Embryos from crossing jam3b^+/-;Tg(ubb:FATC) fish to jam3^+/-;Tg(hsp:cre) fish were heatshocked at 24 hpf. At 48-50 hpf, skeletal muscles in control animals (A-H, jam3b^+/?=jam3b^+/+ or jam3b^+/-) were multinucleated, as visualized by DAPI staining (blue), and showing LIFEACT-GFP (green) expression. By contrast, in jam3b mutants (I-P, jam3b^-/-), most muscles were mononucleated and their nuclei centrally positioned within each myotome. Correspondingly, the numbers of LIFEACT-GFP+ skeletal myocytes were reduced. Q, A few LIFEACT-GFP⁺ (green) myocytes detected in jam3b^-/- embryos were multinucleated, as shown by DAPI staining (blue). Arrowheads point to nuclei of LIFEACT-GFP⁺ cells. Images are maximum intensity projections from 17.5-25 (A-P) and 10 (Q) μm thick confocal stacks of individual animals and shown in lateral views, dorsal to the left, anterior up. 10-12 embryos of each genotypes were examined.

Acknowledgments

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