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Fig. 5
Fluorescence (green/cyan) and transmitted light (grey) reconstructions from an in vivo acquisition of a 5 dpf Tg(mpx:GFP) zebrafish using (a-e) an image relay for standard full-depth of field OPT at NA~0.035 and (f-j) RoI-OPT at full NA with an axial scan range (SR) of 130 μm.
(a,f) MIP of full reconstruction, (b,g) single YZ slice, (c,h) single XZ slice with depth of field and SR respectively indicated by dotted lines, (d,i) fluorescence reconstruction from region indicated by red box and (e,j) corresponding intensity line profile.
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