ZFIN Image: Patra et al., 2017, Fig. 3

Image

Figure Caption

Fig. 3

Endothelial cell distribution in 8 months old mouse and zebrafish ventricles. (A) Representative confocal images of sagittal cryosections through 8 months old mouse hearts stained for α-actinin (green; marking cardiomyocytes), CD31 (red; marking the cell membrane of endothelial cells) and DAPI (blue, staining the nuclei). White arrows and arrowheads point to bigger coronary vessels (visible lumen) and capillaries (lumen not visible) in cardiac tissue, respectively. (B–D) Representative confocal images of sagittal cryosections through 8 mpf Tg(kdrl:Hsa.HRAS-mCherry) zebrafish hearts stained for phalloidin (green; marking cardiac tissue), mCherry (red; marking the cell membrane of endothelial cells) and DAPI (blue; staining the nuclei). White arrows point to bigger coronary vessels (visible lumen). Yellow arrows point to endocardial cells in the cardiac tissue. ‘B’ and ‘C’ images show different optical planes of the same tissue section.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.