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Fig. 3
miR-145 is critical for liver morphogenesis.
Liver morphology at 4 dpf after control mimic (A), miR-145 inhibitor (B) and miR-145 mimic (C) injections in Tg(fabp10:EGFP) embryos. One hundred embryos per experimental group from one clutch were used and three independent replicates were performed. A 3D image of the liver was analyzed using Leica SP5 confocal microscope and Imaris software (D-F). The liver size was examined by measuring the volume of EGFP expression (G). Ten embryos per experimental group were used and three independent replicates were performed. The EGFP expression of Tg (fabp10:EGFP) fish showed an enlarged liver in miR-145 inhibitor-injected fish and an underdeveloped liver in miR-145 mimic-injected fish fish as compared to that in control fish. Scale bars, 100 μm; EGFP, enhanced green fluorescent protein; *P < 0.05, t-test.