ZFIN Image: Li et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Male kras^V12 zebrafish developed more severe liver tumors during long-term tumor induction.

(A) Diagram of experimental design and schedules of sample collection for the long-term tumor induction. (B) Kaplan-Meier survival curve of wt and kras^V12 fish. (C) Quantification of the percentage of multi-nodular tumors (n = 5–8 for each group). (D–F) Representative images and liver histology from female and male wild type and kras^V12 fish at 1.5 mpi (D), 3 mpi (E) and 5 mpi (F). The livers are outlined in dash lines as well as marked by GFP expression in kras^V12 fish. Multi-nodular tumor was observed only in male kras^V12 fish at 3 mpi and 5 mpi. Individual nodules are indicated by GFP expression as shown in inlets for kras^V12 fish and also pointed by arrows in the brightfield images. The multi-nodular tumors at 5 mpi were characterized by mixed types of HCC and HB separated by dash lines in the H&E staining. Scale bars, 2 mm for gross observation pictures and 20 μm for histology images.

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Phenotype details

Acknowledgments

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