ZFIN Image: Just et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Overexpression of tbx20 in wild-type zebrafish embryos results in reduced ventricular cardiomyocyte proliferation.

(A, B) Dissected wild-type hearts injected with KCl (A) and tbx20 mRNA (B) at 72 hpf, stained against MEF-2 (red) after incorporation of 5-ethynyl-2'-deoxyuridine (EdU; green) to visualize the effect of tbx20 overexpression on cardiomyocyte proliferation. (C) Wild-type zebrafish hearts injected with wild-type tbx20 mRNA show significantly reduced numbers of ventricular cardiomyocytes at 72 hpf compared to control injected hearts (wt+KCl: 148.6±2.9; wt + tbx20 mRNA: 64.0±2.017, n = 10, p< 0.0002). (D) Ventricular cardiomyocyte proliferation in wild-type embryos injected with tbx20 mRNA is significantly reduced compared to control injected embryos at 72 hpf (wt+KCl: 8.9 ± 0.38; wt+tbx20 mRNA: 1.1±0.28, n = 10, p = 0.0001).

Acknowledgments

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