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Fig. 4
The ultimate localization of MG-derived progenitors becomes progressively more biased at later rounds of proliferation.
Fish were given an intraperitoneal injection of BrdU at 2 dpi, followed by an injection of EdU at 3, 6, or 12 dpi. Each sample was harvested at 14dpi. (A–C) EdU+ nuclei were counted at the times indicated and the percentage of EdU+ nuclei residing in the (A) ONL, (B) INL and (C) GCL was determined for each injury model. Data represents means ± s.d. (n ≥ 3). *P < 0.02941. (D,E) The percentage of ONL EdU+ nuclei residing in (D) the upper region or (E) the lower region was determined for each injury model. (F) Co-staining samples for BrdU and EdU demonstrates that the cells proliferating at later times are a subpopulation of those proliferating at earlier times. (G–L) Representative images of retinal sections analyzed in (A–F) that were stained for EdU at (G–I) 3 dpi or (J–L) 6 dpi following (G,J) needle poke, (H,K) PA or (I–L) NMDA injury. Scale bar is equal to 50 μm. Abbreviations are as in Fig. 1.