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Fig. 3
Effects of embryonic atrazine exposure on adult female zebrafish.
A representative image of an adult female from the control group (a) compared to an adult female with a swollen abdomen in the 30 ppb developmentally exposed group (b). There was a ~5% incidence rate observed in this treatment group (~24–37 females assessed in each treatment of the four replicates). No significant difference in overall body weight was seen between the control and 30 ppb treatment group (c), but a significant increase in ovary weight was observed (d) (4 replicates with 10 female fish assessed per treatment replicate). Moreover, in comparison to the normal adult female zebrafish (e) an increase in atretic ovarian follicles (black arrow) was observed in those fish that presented with abdominal swelling (f) determined to be from an inability to release eggs (4 replicates with 10 female fish assessed per treatment replicate). Furthermore, while no significant differences were observed in estradiol (g), a significant increase in progesterone (P4) was present in adult females exposed to 3 or 30 ppb atrazine during embryogenesis (h) [4 to 7 adult females were pooled for each biological replicate (n = 4)]. Error bars are expressed as ± SD. (*p < 0.05).