Fig. 7

Fig. 7

Vital dyes. (A) Chemical structures of commonly used cell-permeant dyes. (B) Neuron-like cell stained with DiOC₆ (1 dap). The complete endomembrane system is visible. In a nearby process of a different cell, stained clusters can be observed (white arrowheads). Inset: Corresponding transmitted light image. (C) Striated myocyte co-stained with DiOC₆ and Hoechst33258 (1 dap). (D,E) Striated myocyte, stained with DiOC₆ (2 dap), (E) is a detail of (D). (F) Neuron-like cells stained with Nile Red (1 dap). Membranes are displayed in red, lipid droplets in yellow (white arrowheads). Inset: Corresponding transmitted light image. (G) Striated myocyte stained with LysoTracker (2 dap). (H) Myocyte stained with MitoTracker (2 dap). (I) Neuron-like cell stained with MitoTracker with distinct mitochondria along processes (2 dap). Scale bars, 10 µm. Each staining method was performed three times or more with several wild type cultures.