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Fig. 6
Purkinje cells of 4 dpf embryos in culture. (A) Tg(ca8:FyntagRFP)bz4Tg embryo (dorsal view, 4 dpf). The cerebellum with red fluorescent Purkinje cells shown in (B) is marked by a red box. Scale bar, 100 µm. (C) Decapitated head of a 4 dpf Tg(ca8:FyntagRFP)bz4Tg embryo. Scale bar, 100 µm. (D-H) Red fluorescent Purkinje cells displaying characteristic morphologies at 1 (D,E), 2 (F) and 3 (G,H) dap. Scale bars, 10 µm. The figure shows a collection of the images of two preparations with comparable results.
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Reprinted from Developmental Biology, 430(1), Sassen, W.A., Lehne, F., Russo, G., Wargenau, S., Dübel, S., Köster, R.W., Embryonic zebrafish primary cell culture for transfection and live cellular and subcellular imaging, 18-31, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.