ZFIN Image: Feng et al., 2017, Fig. 6

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Fig. 6

Constitutive photo-activation of kRASG12V expression in localized tissue and single cells. To induce constitutive activation of kRASG12V, Cre-ERT2 mRNA was injected into Tg(ubi:loxP-Eos-stop-loxP-kRASG12V-T2A-mTFP) embryos at the one-cell stage. (A) Despite some minor light independent activation of Cre, mTFP expression could be fully induced by both cyclofen and caged cyclofen photo-activation. (B) Quantification of kRASG12V activation signal via the ratio of the fluorescence of the co-expressed mTFP protein and the (partially) floxed Eos protein. (C) UV (lamp or laser) and two-photon activation were performed at 10hpf to achieve global (with the UV lamp), local (with the UV laser) and single cell (with 2-photon laser) activation. Fish were imaged at 2dpf. Scale bar: 200 μm. Data are presented as mean ± SEM. ***p < 0.001; N.S, not significant.

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