IMAGE

Fig. S1

ID
ZDB-IMAGE-170921-61
Source
Figures for Sedykh et al., 2017
Image
Figure Caption

Fig. S1

Generation and characterization of mutant alleles at zic2a and zic2b loci.

A: zic2agbt133 was identified in an insertional mutagenesis screen (Clark et al., 2011). This insertion in the first coding exon of zic2a, near the N-terminus of the predicted open reading frame of zic2a. This allele is predicted to be a functional null. Tracks from individual embryos deep-sequenced on the Illumina platform show that the insertional allele inhibits transcription of the first coding exon of zic2a. B: CRISPR and TALEN nucleases were designed to target different sites in the first coding exon of zic2b.Two mutant alleles of zic2b were generated using CRISPR mutagenesis (uw1127 and uw1116). A third allele of zic2b was generated using TALEN mutagenesis (uwt104). Uwt104 and uw1116 cause frameshifts; uw1127 is an in-frame insertion that contains a translational termination codon. All three alleles encode truncated proteins that do not contain the ultra-conserved zinc binding domains. C: normal 5 dpf sibling and D: zic2 mutant with cerebral edema (arrow) from a zic2 heterozygous carrier incross.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 429(1), Sedykh, I., Yoon, B., Roberson, L., Moskvin, O., Dewey, C.N., Grinblat, Y., Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis, 92-104, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.