ZFIN Image: Salamaga et al., 2017, Fig. S5

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Figure Caption

Fig. S5

Analysis of the virulence of E. faecalis ΔatlA mutants forming long chains.

A. Comparison of median forward scattered (FSC) light values corresponding to the cell chain lengths of WT (OG1RF), ΔatlA and sonicated ΔatlA (ΔatlA^S) strains. B. Light microscopy images showing cell chain lengths of WT and ΔatlA derivatives expressing cytoplasmic GFP. C. Survival of zebrafish larvae (n>20) following infection with E. faecalis OG1RF (WT) and atlA isogenic deletion mutant before (ΔatlA) and after (ΔatlA^S) sonication to disperse long chains. The results corresponding to three independent experiments are shown. For each experiment, the number of cells injected (determined after sonication) is indicated. D. P values resulting from pairwise comparisons using the log rank test. E. survival of phagocyte-depleted zebrafish larvae following injection with 1250 cells of E. faecalis OG1RF (WT) or ΔatlA.

Acknowledgments

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