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Fig. 4 S1
Delayed macrophage recruitment in zebrafish by Clodronate liposome pre-depletion.
Adult mpeg1.4:mCherry-F;mpx:GFP zebrafish were injected with PBS or clodronate liposomes 1 day before injury. Hearts were collected at 7 dpci, and cryosections stained with GFP antibody to examine neutrophils, and mCherry antibody and IB4 to examine macrophages. Positive cells, both in the injured area itself and within 100 μm of the injured area, were quantified (B; n ≥ 3). Dotted lines delineate the injured area; scale bars, 200 μm. At 1 dpci, macrophage numbers in CL-exposed hearts were significantly lower than in controls, while neutrophils numbers were similar. At 7 dpci, neutrophil numbers were significantly higher in CL-exposed hearts, while macrophage numbers were not significantly different.