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Fig. S2
Related to Figure 2. Development of functional intrinsic and visually induced properties of newborn neurons
(A) Development dynamics of the coefficient of variation of neuronal responses to drifting gratings, for newborn-labeled (red) and mature (blue) neurons (newborn-labeled neurons: n=7, 10, 6 and 3, for 1-4 dpe, respectively; mature neurons: n=973, 1340, 858 and 453 mature neurons, from 5, 7, 4 and 2 larvae). **: p < 0.01. Error bars: SEM
(B-D) Top, example of an optical plane of the optic tectum with neurons color-coded according to their coefficient of variation (B), orientation (C) and direction-selectivity index (D). Magenta: poorly responsive neurons (in C-D, p>0.01, Mann-Whitney U tests or silent neurons in B). Note that these neurons were mainly clustered in close vicinity of the neurogenesis region (dashed area). Neurons with noisy baseline were left unlabeled. Arrows: orientation of the larva: L: left, R: right, A: anterior and P: posterior. Scale bar: 50 μm. Bottom, averaged distributions of the measured parameters on top, across the normalized coordinates along the caudo-rostral axis (white dashed curve on top, C: caudal, R: rostral), for all neurons, from 5 to 8 dpf (n=5046 neurons, from 18 larvae). Confidence interval: SEM. Changes in the orientation-, direction-selectivity index and coefficient of variation were significant across the caudo-rostral axis (p=7.61x10-20, p=4.29x10-23 and p=4.57x10-33, respectively, Kruskal-Wallis tests). Note the large differences for the three measured parameters close to the neurogenesis region (dashed region). Neurons involving few pixels (less than 5) or with unstable baselines (rapid changes generally indicating movement artifacts) were left unlabeled.