ZFIN Image: Ziegler et al., 2017, Fig. 4

Image

Figure Caption

Fig. 4

Increased vascularization and contractility in engineered heart tissue underThymosin 4 treatment. ( a) Cross sections of EHTs fromreporter mice expressing LacZ in endothelial cells (Cdh5-CreERT2 × Rosa26-LacZ) reveal an increase in capillary density if treated with T4. (b and c) AAV-mediated transfection with T4 (rAAV.T4, versus rAAV.GFP as control) of EHTs implanted into skinfold chambers leads to an increase of graft-vascularization, both 5 and 8 days after implantation ( = 4 per group, ∗ < 0.05; ∗∗ < 0.0008). (d) Images of whole skinfold chambers containing EHTs generated fromLacZ reporter mice show little sprouting of graft resident endothelial cells into host tissue, while rAAV.T4 treatment drastically induces angiogenic sprouting into the host tissue of skinfold chambers (dotted lines indicate the graft/host border). (e) Treatment with rAAV.T4 leads to an increase of spontaneous contractions in implanted EHTs compared to rAAV.GFP control ( = 4 per group, ∗ < 0.05).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Stem Cells Int.