ZFIN Image: Wild et al., 2017, Fig. S9

Image

Figure Caption

Fig. S9

Ectopic venous sprouting requires hemodynamic factors and Akt

(a-d) Loss of hemodynamics rescues hyper-branching in flt1^ka601 mutants. (a) WT treated with DMSO; (b) flt1^ka601 treated with DMSO; (c) inhibition of cardiac activity with 2,3-Butanedione monoxime (BDM) in flt1^ka601 (d) flt1^ka601 exposed to 2.5X tricaine. mean ± s.e.m, 20-25 embryos/group, Mann Whitney U test. (e) Quantification of a-d. Note that inhibiting blood flow with BDM or tricaine inhibited ectopic branching. (f) Vascular branching after blockade of PI3 kinase with wortmannin. mean ± s.e.m, 3 experiments, 15-18 embryos/group, Mann Whitney U test. Scale bar, 20μm in a-d.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.