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Fig. S1
LightOn system induces kaede expres-sion from the UAS promoter in a light-gated manner. A-C. Tg(UAS:kaede) embryos were injected with GAVPO mRNA and then illuminated with con-stant, global blue light (465 nm) from approximately 4-24 hpf (hours post-fertilization). GAVPO injected embryos showed robust induction of Kaede fluores-cent protein at 24 hpf (C), with no visible induction of Kaede expression in dark (B) or uninjected controls (A). D-F. Following blue light illumination, GAVTA is unable to induce transcription of kaede from the UAS promoter (F, F').
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