ZFIN Image: Davey et al., 2016, Fig. S9

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Figure Caption

Fig. S9

The effect of PCP on protrusion dynamics is dependent on the migratory environment.

(A) Method used to isolate and identify FBMNs in primary culture. Embryos used were Tg(isl1:mTFP);Tg(hoxb1a:RFP) allowing for the differentiation between FBMNs and other branchiomotor neurons labeled by Tg(isl1:mTFP). (B,C) Cultured Tg(isl1:mTFP); Tg(hoxb1a:RFP) FBMNs from a wild type (B) and a vangl2 mutant embryo (C). (B',C') Time-lapse spinning-disc confocal series of boxed region from B and C. (D) Quantitation of filopodial lifetime for cultured FBMNs. Each timelapse was 600 seconds total. p = 0.9044, n.s. (E) Quantitation of the maximum filopodial length for cultured FBMNs. p = 0.0856, n.s. Wild type: N = 8 neurons, 64 filopodia. vangl2^-/-: N = 8 neurons, 61 filodpodia. Graphs represent data as mean ± SEM. Each data point is the average lifetime (D) or maximum length (E) for all the filopodia of one FBMN. Significance was determined using an unpaired, two-tail t-test with Welch’s correction.

Acknowledgments

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