ZFIN Image: Davey et al., 2016, Fig. S6

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Figure Caption

Fig. S6

Migrating FBMNs display polarized protrusions that fail to polarize in vangl2 mutants.

(A,C,E) Representative frames of mTFP expressing FBMNs from time-lapse images taken at 24 hpf to 32 hpf. (B,D,F) Each raw data point for protrusion angle is plotted on the circular graph below. Each division is 10 degrees. A, anterior. P, posterior. M, medial. L, lateral. Filopodia are radial in wild type FBMNs prior to exiting r4 (A,B, N = 3 embryos, 5 neurons, 28 filopodia) and become polarized to the posterior side of the cell during migration (C,D, N = 8 embryos, 10 neurons (6 in r5 and 4 in r6), 52 filopodia). FBMN protrusions fail to polarize in vangl2 mutants (E,F, N = 5 embryos, 7 neurons, 61 protrusions). Scale bar: 16μm.

Acknowledgments

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