ZFIN Image: Davey et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Post-mitotic FBMNs require PCP signaling for migration.

(A) Schematic of the late stage FBMN transplantation procedure in which a small number (1–5) of post-mitotic, pre-migratory FBMNs are moved from r4 of a Tg(isl1:GFP) donor into r4 of a stage-matched, 16 hpf Tg(isl1:mRFP) host. (B, C) Live confocal images showing dorsal views of chimeras at 48 hpf with anterior to the top. Transplant conditions are indicated as donor→host. Cascade blue-dextran marks all donor-derived cells (blue), Tg(isl1:mRFP) marks host FBMNs (magenta) and Tg(isl1:GFP) marks donor-derived FBMNs (green). White arrows indicate donor-derived FBMNs at 48 hpf. (D) Quantitation of the percent of donor-derived FBMNs at 48 hpf that failed to migrate (r4), partially migrated (r5) or fully migrated (r6). Each histogram refers to the transplant condition in the image to its left and numbers indicate the number of FBMNs represented in each bar. WT→WT, N = 42 embryos, 174 FBMNs; WT→ vangl2^-/-, N = 16 embryos, 73 FBMNs. ***p<0.0001 compared to WT→WT control. Brackets indicate rhombomere location. Scale bar: 50μm.

Acknowledgments

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