ZFIN Image: Perens et al., 2016, Fig. 2 S2

Image

Figure Caption

Fig. 2 S2

Comparable proliferation in the IM of wild-type and han^s6 mutant embryos.

(A, B) Immunofluorescence for Pax2a and phospho-Histone H3 (pH3) in wild-type (A) and han^s6 mutant (B) embryos at the 6 somite stage; dorsal views, anterior to the left, are three-dimensional reconstructions of 400 µm long regions used for quantification of the numbers of Pax2a⁺ (A', B') and pH3⁺ (A'', B'') cells. Arrows indicate Pax2a⁺ pH3⁺ cells. Scale bar represents 50 μm. (C) Table compares the number of Pax2a⁺ pH3⁺ cells per 100 µm of IM, the number of Pax2a⁺ cells per 100 µm of IM, and the proliferation index (Pax2a⁺ pH3⁺ cells / Pax2a⁺ cells X 100) in wild-type and han^s6 mutant embryos. Data shown represent the average and standard deviation for each value. While there was a statistically significant increase in the number of Pax2a⁺ cells in han^s6 mutants compared to wild-type (p<0.0001), no significant differences were observed for the number of Pax2a⁺ pH3⁺ cells (p=0.1461) or the proliferation index (p=0.0654).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife