ZFIN Image: Marro et al., 2016, Fig. S3

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Figure Caption

Fig. S3

col12a1a-expressing cells accumulate at the site of injury at the onset of heart regeneration.

(A-K) In-situ hybridization of heart sections at early time points after cryoinjury with probes of two col12 paralogs (purple) followed by immunofluorescence staining against Tropomyosin (blue) and Col XII (green). (A-D) Uninjured hearts do not markedly express col12a1a, but col12a1b in the epicardium, which is surrounded by the Col XII deposition. (E-G) At 1 dpci, col12a1a-expressing cells cover the circumference of the injured heart, Col XII persists at the epicardium at the side of injury. (I-L) At 4 dpci, col12a1a- and col12a1b-expressing cells accumulate at the surface of the injury site. The gene expression correlates with the localization of Col XII protein.

Acknowledgments

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